Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (IsoT), GenBank Accession No. NM_001098536, a.a. 2-858(end) with an N-terminal FLAG-tag, MW=96 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

P45974

USP 5, Ubiquitin-Specific Protease 5, Isopeptidase T, IsoT, USP-5

14.3 pmole/min/µg

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP5 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT, and 90 ?g/ml FLAG peptide

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Dayal, S., et al. (2009). J. Biol. Chem. 284(8):5030-41.
2. Reyes-Turcu, F.E., et al. (2008). J. Biol. Chem. 283(28):19581-92.

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein