Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 259-605(end) with an N-terminal His-tag, MW=40.9 kDa, expressed in an E. coli cell expression system.

O75604

USP 2, Ubiquitin-Specific Protease 2, UPB41, USP-2

247 pmol/min/µg

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 2.2 mM KCl, 15 mM ?-mercaptoethanol, 250 mM imidazole, and 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Stevenson, L.F., et al. (2010). EMBO J. 140(3): 384-96.
2. Priolo, C., et al. (2006). Cancer Res. 66(17): 8625-32.
3. Haimerl, F., et al. (2009). J. Biol. Chem. 284(1): 495-504.

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein