Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), GenBank Accession No. NM_005151, a.a. 2-494(end) with an N-terminal FLAG-tag, MW=57 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

P54578

USP 14, Ubiquitin-Specific Protease 14, TGT, tRNA-guanine transglycosylase, USP-14

5.7 pmole/min/µg

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP14 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Chen, P.C., et al. (2009). J. Neurosci. 29(35):10909-19.
2. Chen, Y.C., et al. (2010). RNA 16(5):958-68.
3. Nagai, A., et al. (2009). Biochem. Biophys. Res. Commun. 379(4):995-1000.

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein