Human ubiquitin specific peptidase 9X, also known as DFFRX, FAF, FAM, and MRX99, GenBank Accession No. NM_003470, a.a. 2 – 2554 (end) with N-terminal His-Tag and C-terminal FLAG-Tag, expressed in a HEK293 cell expression system. MW = 293 kDa.

Q93008

DFFRX, FAF, FAM, and MRX99

Aqueous buffer solution.

70 pmol/min/μg

50mM Tris-HCl, 0.5mM EDTA, 0.05% Tween 20, 1mM DTT, 500nM Ubiquitin-AMC. Incubate at room temperature for 30 minutes. Fluorescence intensity is measured at exc/em of 360nm/460nm using Tecan M1000 plate reader.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 μg/ml FLAG peptide, 20% glycerol.

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

1. Schwickart, M., et al., Nature. 2010 Jan; 463: 103-107.
2. Dupont, S., et al.,Cell. 2009 Jan. 136(1): 13-14.

Avoid freeze/thaw cycles.

Ubiquitin

Protein