Human ubiquitin specific peptidase 7 (USP7) or HAUSP, GenBank Accession No. NM_003470, a.a. 2 – 1102 (end) with N-terminal His- Flag tag, MW = 130 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Q93009

Ubiquitin specific peptidase 7, USP 7, HAUSP, USP-7

545 pmole/min/µg

Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT. 0.5 mM EDTA, and 500 nM Ub-AMC. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.

47.5 mM Tris-HCl, pH 8.0, 131 mM NaCl, 2.6 mM KCl, 90 mM imidazole, 5% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Fernandez-Montalvan, A., et al., FEBS J. 2007 Aug;274(16):4256-70.
2. Li, M., et al., Mol Cell. 2004 Mar 26;13(6):879-86.

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein