Human USP10 (Ubiquitin-Specific Protease 10) or Ubiquitin C-terminal Hydrolase 10, GenBank Accession No. NM_005153, a.a. 2-798(end) with an N-terminal FLAG-tag, MW=88 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Q14694

USP 10, Ubiquitin-Specific Protease 10, Ubiquitin C-terminal Hydrolase 10, Ubch10, USP-10

1.5 pmole/min/µg

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP10 in a 50 µL volume. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Faus, H., et al. (2005). Mol. Cell. Endocrinol. 245(1-2):138-46.
2. Soncini, C., et al. (2001). Oncogene 20(29):3869-79.
3. Yuan, J., et al. (2010). Cell 140(3): 384-96.

Application Reference:
Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1 (2014)

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein