UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002), a.a. 2-231(end), with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli.

P15374

UCHL3, Ubiquitin Carboxy-terminal Hydrolase L3, Ubiquitin C-terminal Hydrolase L3

20,733 pmole/min/µg

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL3 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation 340 nm, emission = 460 nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Wada,  H.,  et  al.  (1998).  Biochem.  Biophys. 
     Res. Commun. 251 (3): 688–92. 
2. Nam, M.J., et al.  (2004). Proteomics 3  (11): 
     2108–15. 
3. Setsuie,  R.,  et  al.  (2009).  Neurochem  Int. 
     54(5-6):314-21. 

Avoid freeze/thaw cycles

Ubiquitin Hydrolase

Recombinant Protein