UbcH6, also known as UBE2E1 (GenBank Accession No. NM_003341), a.a. 2-end, with N-terminal His-tag, MW = 22 kDa, expressed in an E. coli cell expression system.

P51965

UbcH6, UBE2E1

50 µl reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, UBE1 as an E1 (0.5 µg), UbcH6, Biotin-Ubiquitin (0.5 µg) and Smurf2 as an E3(2 µg) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, then Europium-labeled secondary antibody, and detect by time resolved fluorescence (λex=330 nm, λem=620 nm).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

>6 months at -80°C.

Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sommer, T., et al. 1993. Nature 365 (6442):176-9.
2. Yang, M. et al. 1997. J. Biol. Chem. 272 : 1970-1975.

Avoid freeze/thaw cycles

Ubiquitin Conjugating Enzyme (E2)

Recombinant Protein