UBCH13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze the formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBCH13 is required for signaling by a variety of receptors important in immune regulation, making it a candidate target for inflammatory diseases. UBCH13 is also critical for double-strand DNA repair and thus a potential radiosensitizer and chemosensitizer target for oncology. The UBCH13 TR-FRET Assay Kit is designed to measure UBCH13 ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and a terbium-labeled anti-His antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

UBE2N, HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, ubiquitin conjugating enzyme E2 N

See assay protocol for detailed instructions.

At least 6 months from date of receipt when stored as directed.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Madiraju, C., et al., J. Biomol. Screen. 2012;17(2):163-76.

Avoid multiple freeze/thaw cycles.

Ubiquitin

Assay Kit