| 产品名称 | SENP2, His-tag |
| 产品货号 | 81095 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 5000 units |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 28 kDa
Tag(s): N-terminal His-tag
Background: SUMO Protease 2, a highly active and robust recombinant protease, cleaves hSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3. As a result, SUMO Protease 2 will not cleave within the fused protein of interest.
Description: Human SENP2 recombinant protein, with N-terminal His-tag, expressed in E. coli.
UniProt Q9HC62
Synonym(s): SUMO Protease 2
Purity: ≥95% by SDS-PAGE and RP-HPLC
Supplied As: 10U/µl in aqueous buffer
Specific Activity: ≥105 Units/mg
Unit Definition: 1 Unit of SUMO Protease 2 cleaves >90% of 100 µg SUMO Protease 2 Control Protein in 1 hour (37°C)
Formulation: 50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol
Format: Aqueous buffer solution
Storage / Stability: Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C. Application(s): Useful for removal of SUMO-tags from recombinant SUMO fusion proteins
Reference(s): 1. MarblestoneJG, et al. (2006). "Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO," Protein Science, 15:182-9.
2. Butt TR, Edavettal SC, Hall JP, Mattern MR (2005). "SUMO fusion technology for difficult-to-express proteins," Protein Expr Purif., 43(1):1-9. 3. Zuo X, et al. (2005). "Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.," J. Struct. Funct. Gen., 6:103-11. 4. Malakhov MP, et al. (2004). "SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins," J. Struct. Funct.Gen., 5:75-86. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Ubiquitination (SUMOylation)
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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