| 产品名称 | Praja1, SUMO-tag |
| 产品货号 | 81055 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 25 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 71 kDa
Genbank #: NP_660095
Tag(s): N-terminal SUMO-tag
a.a: full length
Background: Praja1 (along with Praja2, 53% identity) is a RING-H2 domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. Substrates for Praja1 include Dlxin-1 (MAGE-D1, human) which regulates Dlx5 dependent transcriptional function (involved in osteoblast differentiation) and ELF and Smad3 involved in the TGF-beta signaling pathway implicated in gastrointestinal cancers.
Description: Human Praja1 recombinant protein (Genbank Accession No. NP_660095), full length with N-terminal SUMO-tag, expressed in E. coli.
UniProt Q8NG27
Synonym(s): RNF70, RING finger protein 70
Purity: ≥85% by SDS-PAGE
Biological Activity: Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Formulation: 50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Format: Aqueous buffer solution
Storage / Stability: Stable for ≥1 year at –80°C. Application(s): Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Reference(s): 1. Yu, P., et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics, 2002. 79(6): p. 869-74.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9. 3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705. 4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Ubiquitination (Ubiquitin Ligase (E3))
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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