LDN-57444 is a cell permeable ubiquitin C-terminal hydrolase (UCH-L1) inhibitor (Ki=0.4 μM). Decreases proteasome activity and increases levels of ubiquitinated proteins. Induces apoptosis and causes dramatic alterations in synaptic protein distribution and spine morphology in vivo. LDN-57444 is a useful tool to study UCH-L1 in different pathological conditions such as cancer and neurodegeneration.

1H–Indole–2,3–dione, 5–chloro–1–[(2,5–dichlorophenyl)methyl]–, 3–(O–acetyloxime)

≥98% by TLC.

Soluble in DMSO (20 mg/ml)

Yellow powder

Store at +4°C. Stable as supplied for up to 2 years when stored dessicated at +4°C. Store solutions at -20°C for up to 1 week.

Useful for the inhibition of Ubiquitin C-terminal Hydrolase (UCH-L1) in drug discovery studies.

1. Tan, et al Endoplasmic reticulum stress contributes to the cell death induced by UCH-L1 inhibitor. Mol.Cell.Biochem. 318:109 (2008).
2. Susor, A., et al., Proteomic analysis of porcine oocytes during in vitro maturation reveals essential role for the ubiquitin C-terminal hydrolase-L1. Reprod. 134:559 - 568, (2007) .

Protect from moisture. Avoid freeze/thaw cycles.

Ubiquitin Enzymes

Inhibitors/Activators