Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. SHP-2 (PTPN11) is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

The SHP-2 (catalytic domain) Homogeneous Assay Kit is a complete assay system designed to measure SHP-2 activity for screening and profiling applications. Using this kit, only one simple step on a microtiter plate is needed to analyze the SHP-2 activity level. The fluorogenic substrate, DiFMUP, is incubated with purified SHP-2 and the enzymatic activity releases DiFMU fluorophore that can then be measured using a fluorescence reader.

SHP2, CFC, NS1, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, MGC14433, SH2, SH 2, SH-2, SHP, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen, shp

Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SHP-2 activity measurements. In addition, the kit includes purified SHP-2 enzyme and SHP-2 inhibitor, orthovanadate, for use as a positive and negative control.

CatalogNumber	Component	Amount	Storage
30022	Recombinant Human SHP-2	1 µg	-80°C	Avoid freeze/ thaw cycles!
79769	1mM SHP-2 Substrate	50 µl	-80°C
79626	5X SHP-2 Assay Buffer	3 ml	-20°C
	1mM Na3VO4	20 μl	-80°C
79685	black, low binding black microtiter plate	1	Room Temperature

See assay kit data sheet for detailed protocol.

Great for studying enzyme kinetics and HTS applications.

Chai, J., et al., Cell, 2001 Mar 9;104(5):769-80.
Denault, JB., and Salvesen, GS., J. Biol. Chem., 2003 Sep 5;278(36):34042-50.

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Protein phosphatase

Assay Kit