Human matrix metallopeptidase-9 (MMP9), also known as gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB and EC 3.4.24, with Gln to Arg mutation on a.a. 279, GenBank Accession No. NM_004994), a.a. 20-707 (end) with C-terminal His-tag, MW=77.8 kDa, expressed in an HEK293 cell expression system.

P14780

matrix metallopeptidase-9, MMP9, gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB, EC 3.4.24, MMP

≥ 2500 pmole/min/µg

Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/ml in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37ºC for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.

Aqueous buffer solution

>6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Wenham, R.M., et al., J. Soc. Gynecol. Investig. 2003 Sep;10(6):381-7.
2. Li, X., et al., J. Cancer Res. Clin. Oncol. 2013 Sep;139(9):1433-47.

Avoid freeze/thaw cycles.

Protease

Recombinant Protein