Human rhinovirus 3C protease (HRV 3C Protease) with Nterminal His-GST-tags (can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.), Recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. Cleaves between Gln and Gly. MW = 47 kDa in E. coli expression system.

Human rhinovirus 3C protease, HRV3C

1 Unit of HRV 3C Protease cleaves >95% of 100 μg of target protein at 4°C for 16 hours.

A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with HRV3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM β-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.

50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM TCEP, 50% glycerol

Aqueous buffer solution

>6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Timmer JC, Salvesen GS. Methods Mol Biol. 2011;753:243-55.

Avoid freeze/thaw cycles.

Protease

Recombinant Protein