Hepatitis C virus (HCV) genotype 2a, also known as HCV2a, is a fusion protein of a.a. 21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3 (GenBank Accession No. FJ393024) with N-terminal FLAG-His-tag, MW = 22.3 kDa, expressed in an E. coli expression system.

B8XJX9

hepatitis c virus genotype 2a

93 pmole/min/µg

Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min at ex350/em500.

40 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.2% Triton X-100, 10 mM ß-mercaptoethanol, 100 µg/ml FLAG peptide, and 30% glycerol

Aqueous buffer solution

>6 months at -80°C.

1. Jensen, T.B., et al., J. Infect. Dis. 2008;198(12):1756-1765.
2. Wang, Y., et al., Cell Immunol. 2006 May;241(1):38-46.

Avoid freeze/thaw cycles

Protease

Recombinant Protein