Human matrix metallopeptidase-1 (MMP1), also known as CLG, Interstitial Collagenase, Fibroblast Collagenase, and EC 3.4.24.7 (GenBank Accession No. NM_002421), a.a. 20-469 (end), with C-terminal His-tag, MW=53 kDa, expressed in an HEK293 cell expression system.

P03956

matrix metallopeptidase-1 , MMP1, CLG, Interstitial Collagenase, Fibroblast Collagenase, EC 3.4.24.7, MMP

≥ 105 pmole/min/µg

Activation: This is a proform enzyme and requires activation prior to testing activity. Dilute the enzyme to 100 ng/ml in a solution containing 50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05% Brij-35, and 1 mM APMA (amino-phenyl mercuric acetate). Incubate at 37ºC for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.

Aqueous buffer solution

>6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Wenham, R.M., et al., J. Soc. Gynecol. Investig. 2003 Sep;10(6):381-7.
2. Li, X., et al., J. Cancer Res. Clin. Oncol. 2013 Sep;139(9):1433-47.

Avoid freeze/thaw cycles.

Protease

Recombinant Protein