Human insulin-degrading enzyme (IDE), also known as insulysin, Abeta-degrading protease, insulin protease, and insulinase, GenBank Accession No. NM_004969, a.a. 42-1019(end) with N-terminal His-tag, expressed in a Baculovirus Sf9 cell expression system. MW = 114 kDa.

P14735

ide, insulin-degrading enzyme (IDE), insulysin, Abeta-degrading protease, insulin protease, insulinase

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 20% glycerol, 3 mM DTT, and 105 mM imidazole

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Farris, W., et al., Proc. Natl. Acad. Sci. (USA). 2003; 100(7): 4162-4167.
2. Qiu, W.Q., et al., J. Biol. Chem. 1998; 273: 32730-37238.

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Proteases

Recombinant protein