The PARP7 Chemiluminescent Activity Assay Kit is designed to measure PARP7 activity for screening and profiling applications. PARP7 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 384-well plate. Next, the PARP7 biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

TCDD Inducible Poly(ADP-Ribose) Polymerase, ADP-Ribosyltransferase Diphtheria Toxin-Like 14,TCDD-Inducible Poly [ADP-Ribose] Polymerase, Poly [ADP-Ribose] Polymerase

The PARP7 assay kit comes in a convenient 384-well format, with purified PARP7 enzyme, histone mixture and PARP7 assay buffer for 400 enzyme reactions. The key to the PARP7 Chemiluminescent Activity Assay is the biotinylated substrate

See assay protocol for detailed instruction.

Up to 1 year when stored as recommended.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Avoid multiple freeze/thaw cycles.

PARP

Assay Kit