Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal FLAG-Avi-tag™, MW = 117 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

P09874

poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1

Enzyme reaction is conducted using BPS PARP Assay Kit (Catalog Number 80551) at room temperature for 1 hour.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 20% glycerol, 0.2mM TCEP, 80 μg/ml FLAG peptide

Aqueous buffer solution

At least 6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Aguilar-Quesada R, et al., Curr Med Chem. 2007;14(11):1179-87.
2. Pacher P. and Szabó C. Cardiovasc Drug Rev. 2007 Fall;25(3):235-60.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

PARPS

Recombinant Protein