TNKS1 (TRF1-interacting ankyrin-related ADP-ribose polymerase 1), also known as PARP5A, TANK1, or tanykyrase-1 (GenBank Accession No. NM_003747), a.a. 1001-1327(end), with N-terminal GST-tag, MW=62.5 kDa, expressed in Sf9 cells via a baculovirus expression system.

O95271

TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5A, TRF1-interacting ankyrin-related ADP-ribose polymerase 1, TANK1, tanykyrase-1

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Enzyme reaction is conducted using BPS's TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+), Catalog # 80573.

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Chang S. (2010). Aging (Albany NY). 2(10):639-42.
2. Karner, C.M. et al. (2010). Dev Dyn. 239(7):2014-23.
3. McCabe, N, et al. (2009). Oncogene. 28(11):1465-70.

Avoid freeze/thaw cycles.

PARP

Recombinant Protein