Mouse PDE11A (GenBank Accession No. NM_001081033), a.a. 2-933(end) with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

PDE11, phosphodiesterase 11A

≥ 1,560 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE11A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM Glutathione, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Fawcett, L. et al., PNAS. 97(7), 3207-3707 (2000).

2. Horvath, A. et al., Nature Genetics. 38, 794-800 (2006).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein