Human tyrosyl DNA phosphodiesterase 2, or TDP2, (GenBank Accession No. NM_016614), full length with N-terminal His-GST-tag, MW = 67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

PDE, tyrosyl DNA phosphodiesterase2

≥ 186 pmol/min/µg

Enzyme reaction was performed in a reaction buffer (200 µl) containing 50 mM Tris (pH 7.4), 1 mM MgCl₂, 50 mM KCl, 1 mM DTT, 0.1 mg/ml BSA, 2 mM T5PNP and 600 ng TDP2. Reaction was continuously monitored at 415 nm at room temperature for 20 min. Specific activity was calculated based on the molar extinction coefficient of the product (p-nitrophenol), e = 15,000 M^-1cm^-1

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol and 3 mM DTT

Aqueous buffer solution

>6 months at -80°C.

1. Loughney, K, et al., Int. J. Impot. Res.2005, 17(4):320-5
2. Weeks, JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9

Avoid freeze/thaw cycles.

PDE

Recombinant Protein