Rat PDE7A (GenBank Accession No. NM_031080), a.a. 147-482(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

F1LM88

PDE7A, phosphodiesterase 7A

≥354 pmol/min/µg.

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using BPS's PDE7A kit, Cat. # 60370. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of rPDE7A at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Han, P., et al. (1998). Genomics Mar 1;48(2):275-6.
2. Yang, G., et al. (2003). J. Immunol. Dec 15;171(12):6414-20.
3. Yamamoto, et al. (2006) Eur. J. Pharmacol. Jul 10;541(1-2):106-14.

Avoid freeze/thaw cycles.

PDE

Recombinant Protein