Human PDE4D3 (GenBank Accession No. NM_006203), full length, with N-terminal GST-tag, MW=102.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q08499

PDE4D3, phosphodiesterase 4D3

≥ 12,900 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4D3 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Fidani,L., et al., Eur. J. Neurol. 14 (7), 745-749 (2007).
2. Baillie,G.S., et al., Biochem. J. 404 (1), 71-80 (2007).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein