Dog phosphodiesterase 4D (PDE4D) GenBank Accession No. XP_003433853, a.a. 2-679 (end), with N-terminal GST-tag, MW 104 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

F1PKQ9

PDE4D, phosphodiesterase 4D

≥ 5665 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4D at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT

Aqueous buffer solution

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Lynex, C.N., et al., Cell Signal. 2008 Dec,20(12):2247-55.
2. Smith, C.J., et al., Circulation. 1997 Nov 4,96(9):36116-23.

Avoid freeze/thaw cycles

PDE

Recombinant Protein