Human PDE4C1 (GenBank Accession No. NM_000923), full length, with N-terminal GST-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q08493

PDE4C, phosphodiesterase 4C

≥3,000 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4C1 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Wang H. et al., Biochem J. 2007; 408(2):193-201.
2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.

Avoid freeze/thaw cycles.

PDE

Recombinant Protein