Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST-tag, MW= 109 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q07343

PDE4B1, phosphodiesterase 4B1

≥ 8532 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B1 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Shah,V.O., et al., Am. J. Kidney Dis. 48 (4), 616-628 (2006)
2. Smith,P.G., et al., Blood 105 (1), 308-316 (2005).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein