| 产品名称 | PDE4D3 Assay Kit - Assay Kits - Phosphodiesterase(s) |
| 产品货号 | 60346 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle contractility, and
has been identified as a potential risk predictor for ischemic stroke. Additionally, PDE4D has
been associated with asthma pathophysiology and bone formation. The PDE4D gene
encodes at least 9 different isoforms. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled
cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide
product. This increases the size of the nucleotide relative to unreacted cyclic
monophosphate. In the polarization assay, dye molecules with absorption transition
vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes
attached to the rapidly-rotating cyclic monophosphates will obtain random orientations
and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead
complexes will not have time to reorient and therefore will emit highly polarized light.
Description: The PDE4D3 Assay Kit is designed for identification of
inhibitors of PDE4D3 using fluorescence polarization. The assay is based on the binding of
a fluorescent nucleotide monophosphate generated by PDE4D3 to the binding agent. The key to the PDE4D3 Assay Kit is the specific binding
agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4D3
reactions. First, the fluorescently labeled cAMP is incubated with a sample containing
PDE4D3 for 1 hour. Second, a binding agent is added to the reaction mix to produce a
change in fluorescent polarization that can then be measured using a fluorescence reader
equipped for the measurement of fluorescence polarization.
Synonym(s): inhibitor screening, assay kit, PDE4D
Supplied As: The PDE4D3 Assay Kit comes in a convenient 96-well format, with purified PDE4D3enzyme, fluorescently labeled PDE4D substrate (cAMP), binding agent, and PDE assaybuffer for 100 enzyme reactions.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s): Chandrasekaran A, et al., Cell Signal. 2008; 20(1): 139-53.
Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization Warning(s): Avoid freeze/thaw cycles
Scientific Category: PDE
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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