The PDE4C1 Assay Kit is designed for identification of PDE4C1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4C1 to the binding agent. The key to the PDE4C1 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4C1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4C1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.

The PDE4C1 inhibitor screening assay kit comes in a convenient 384-well format, including purified PDE4C1 enzyme, fluorescently labeled PDE4C1 substrate (cAMP), binding agent, and PDE assay buffer for 400 enzyme reactions.

See assay kit data sheet for detailed protocol.

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Chandrasekaran, A., et al., Cell Signal. 2008; 20(1):139-53.

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization

Avoid freeze/thaw cycles.

Phosphodiesterase

Assay Kit