Human PDE4B3 (GenBank Accession No. NM_001037340), 2-721 (end), with N-terminal GST tag, MW=108 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

10,500 pmole/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE4B3 at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

At least 6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Ahmed, T., et al., Neuroscience. 117(3), 627-638 (2003).

2. Millar, J, et al., Science. 310(5751), 1187-1191 (2005).

Avoid freeze/thaw cycles

Phosphodiesterase

Enzyme