Mouse PDE3A (GenBank Accession No. NM_018779), amino acids 484-1141 end), with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

PDE3A, phosphodiesterase 3A, PDE3

≥ 280 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of Mouse PDE3A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 18 mM Glutathione

Aqueous buffer solution

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)

2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Avoid freeze/thaw cycles.

PDE

Recombinant Protein