| 产品名称 | PDE10A (Mouse) Assay Kit |
| 产品货号 | 79643 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Phosphodiesterases (PDEs) play an important role in the dynamic
regulation of cAMP and cGMP signaling. PDE10A is a dual substrate PDE highly
expressed in striatal medium spiny neurons. PDE10A inhibitors can improve the
cognitive symptoms of schizophrenia, and exhibit potential therapeutic value for
Huntington’s Disease. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled
cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide
product. This increases the size of the nucleotide relative to unreacted cyclic
monophosphate. In the polarization assay, dye molecules with absorption transition
vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes
attached to the rapidly-rotating cyclic monophosphates will obtain random orientations
and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead
complexes will not have time to reorient and therefore will emit highly polarized light.
Description: The Mouse PDE10A Assay Kit is designed for identification of inhibitors of Mouse PDE10A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE10A to the binding agent. The key to the Mouse PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE10A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization. Materials Required But Not Provided: Fluorescent microplate reader capable to measure fluorescence polarization. Adjustable micropipettor and sterile tips. 1,4-Dithiothreitol (DTT) 1 M in anhydrous DMSO. Synonym(s): inhibitor screening, assay kit, PDE10A mouse
Supplied As: The Mouse PDE10A Assay Kit comes in a convenient 96-well format, with purified Mouse PDE10A enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s): 1. Narayanan, D.L., Deshpande, D., Das Bhowmik, A., Varma, D.R., Dalal, A. 2018. Familial choreoathetosis due to novel heterozygous mutation in PDE10A. Am J Med Genet Part A. 176A:146–150. 2. MacMullen, C.M., Fallahi, M., Davis, R.L.. 2017. Novel PDE10A transcript diversity in the human striatum: Insights into gene complexity, conservation and regulation. Gene, 606:,17-24. Warning(s): Avoid freeze/thaw cycles
Scientific Category: PDE
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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