Native nucleosome purified from human HeLa cells.

10 mM Tris-HCl, pH 7.2, 5 mM NaCl, 1 mM MgCl2, 0.5 mM CaCl2, 125 mM EDTA, and 10 mM sodium butyrate.

Aqueous solution sufficient for 50 reactions in a 96- well format

At least 6 months at –80°C.

Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.

1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

Application Reference(s):
1. Rapid generation of drug-resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis (2017)
2. One-pot refolding of core histones from bacterial inclusion bodies allows rapid reconstitution of histone octamer (2015)
3. Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity (2015)

The concentration of this product is determined by Bradford and represents only the protein concentration.

Avoid freeze/thaw cycles.

Methyltransferase/Substrate

Purified Protein