Human Histone 4 (HIST2H4A) (GenBank Accession No. NM_003548), a.a. 2-103(end) with N-terminal His-tag, MW = 12.1 kDa, expressed in an E. coli expression system.

P62805

H4, HIST4, Histone h4, HIST2H4A

8 mM PBS pH 7.4, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol.

Aqueous buffer solution

≥ 6 months at -80°C

Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.

1. Kornberg, R.D. (1977) Annu. Rev. Biochem., 46, 931-954.
2. van Holde, K.E. (1989) Chromatin, 1-497.

Application Reference(s):
1. Small Molecule Inhibitors of the Human Histone Lysine Methyltransferase NSD2 / WHSC1 / MMSET Identified from a Quantitative High-Throughput Screen with Nucleosome Substrate (2017)
2.Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus (2013)
3. Extracellular histones are essential effectors of C5aR- and C5L2-mediated tissue damage and inflammation in acute lung injury (2013)

Avoid freeze/thaw cycles

Methyltransferase/Substrate

Recombinant Protein