Human SUV39H1, GenBank Accession No. NM_003173, a.a. 82-412, with N-terminal GST-tag, MW= 64 kDa, expressed in an E. coli expression system.

O43463

KMT1A

≥40%

≥0.1 pmol/min/µg

50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and 10-100 ng SUV39H1) is added to the wells of a substrate-coated Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kang, M.Y. et al., Int. J. Cancer 121, 10, 2192-2197, 2007.
2. Spensberger, D. and Delwel, R. FEBS Lett. 582, 18, 2761-2767, 2008.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Methyltransferase

Recombinant Protein