Human SET and MYNDcontaining protein 4 (SMYD4), also known as KIAA1936, amino acids 2- 804(end), with N-terminal FLAG-tag GenBank Accession No. NM_052928, MW = 90 kDa. This protein is expressed in a Baculovirus infected Sf9 cell expression system.

Q8IYR2

SET and MYND domain-containing protein 4, KIAA1936

Add 50 µl reaction mix (100 M S-adenosylmethionine, SMYD4 enzyme, and HMT buffer 7Z’ (BPS #52193Z) to wells coated with the substrate. Incubate 3-16 h. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 20% glycerol, 0.2 mM TCEP, 80 μg/ml FLAG peptide

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended.

Methyltransferase

Recombinant Protein