Human SMYD2, also known as KMT3C, SET And MYND Domain Containing 2 and Lysine N-Methyltransferase 3C, GenBank Accession No. NM_020197, a.a. 2-433(end) with N-terminal FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW= 51 kDa.

Q9NRG4

KMT3C, SET And MYND Domain Containing 2, Lysine N-Methyltransferase 3C

Aqueous buffer solution.

.004 pmole/min/µg

For standard, H3, 21-44 K36 (1Me)-Biotin was coated onto a neutravadin plate at varying concentrations. For test lots, H3,21-44-Biotin was coated onto a neutravadin plate at 0.4 μg/ml. Reaction mixture contained 50 mM Tris buffer pH 8.8, 5 mM MgCl2, 1 mM TCEP, 0.05% Tween-20, 10 μM S-adenosylmethionine, and various amounts of SMYD2—incubated for overnight at room temperature. Followed by 1 hour incubation with primary antibody against H3 K36 (1Me), and 30 minute incubation with secondary antibody. Luminescence was developed with HRP substrate A and B (Pierce).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 0.2mM TCEP, 80 µg/ml FLAG peptide

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Abu-Farha M. et al., Mol. Cell Proteomics 7(3): 560-572 (2008).
2. Brown M.A. et al., Mol. Cancer 5: 26 (2006).

Avoid multiple freeze/thaw cycles.

Methyltransferase

Recombinant Protein