| 产品名称 | Set7/Set9, GST-tag (E. coli-derived) - Enzymes - Methyltransferase(s) |
| 产品货号 | 51010 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 100 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 61 kDa
Genbank #: NM_030648
Tag(s): N-terminal GST-tag
a.a: 52–266 (end)
Background: SETD7 or SET domain containing lysine methyltransferase 7 is a lysine methyltransferase which can methylates lysine-4 (K4) in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300. SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. SETD7 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site.
Description: Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal GST-tag, MW = 61 kDa, expressed in an E. coli expression system.
UniProt Q8WTS6
Synonym(s): Set7/9, SETD7, KIAA1717, KMT7, SET7, SET9
Specific Activity: 0.58 pmol/min/µg
Assay Conditions: 50 µl reaction mix (50 mM Tris pH8.8, 5 mM MgCl2, 4 mM DTT, 20 µM S-adenosyl-methionine, and 0-10 ng SET7/9) add to the wells coated with the substrate. Incubate at room temp for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then incubate 30 mins with HRP-labeled secondary antibody. Finally, add HRP chemiluminescent substrates and read luminescence.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Ivanov G.S. et al., Mol. Cell. Biol. 27 (19), 6756-6769 (2007). Application Reference(s):
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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