| 产品名称 | G9a (EHMT2), GST-tag (E. coli-derived) - Enzymes - Methyltransferase(s) |
| 产品货号 | 51000 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 50 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 74.6 kDa
Genbank #: NM_006709
Tag(s): N-terminal GST-tag
a.a: 785–1210
Background: EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1) (1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation (2). EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
Description: Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in an E. coli expression system.
UniProt Q96KQ7
Synonym(s): G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2
Specific Activity: 22 pmol/min/µg
Assay Conditions: Enzyme was incubated
with 0.5 µM of biotinylated H3 peptide with
40 µM AdoMet in HMT buffer 1A in a 10-µl
reaction. 5 µl of Anti-Mouse AlphaLISA
acceptor beads (1:250) and 5 µl of Primary
Antibody 7 (1:50) were added and
incubation continued for 30 min.
Streptavidin Donor beads (1:125) were
added (10 µl) and mixture was further
incubated for 15 min at RT before counts
were measured.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007. Application Reference(s):
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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