The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2 activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

KMT3C

The SMYD2 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 substrate, antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase

DMSO >1%, strong acids or bases, ionic detergents, high salt

See product datasheet for detailed assay protocol.

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

Brown MA, et al. Mol. Cancer 2006; 5:26.

MATERIALS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit