The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Q15047

Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET)

Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase buffer, and purified enzyme

See assay kit data sheet for detailed protocol.

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

MATERIALS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Avoid freeze/thaw cycles.

Methyltransferases

Assay Kits