The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

PRMT1, HRMT1L2, IR1B4, HCP1

Kit comes in a convenient format, with 8-well strips precoated with histone H4 peptide substrate, reagents, and purified PRMT1 enzyme for 96 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Dillon S.C., et al. 2005. Genome Biology 6:227.

Application Reference(s):
1. Synthesis and evaluation of protein arginine N-methyltransferase inhibitors designed to simultaneously occupy both substrate binding sites (2015)
2. Arginine Methyltransferase 1 in the Nucleus Accumbens Regulates Behavioral Effects of Cocaine (2015)

* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit