The NSD1 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD1 activity for screening and profiling applications. The key to the NSD1 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Nuclear Receptor Binding SET Domain Protein 1, KMT3B, NSD1, NSD

Kit comes in a convenient format, with 96-well plate precoated with histone H3 substrate, reagents, and purified NSD1 enzyme for 96 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Dillon S.C., et al. 2005. Genome Biology 6:227.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit