The Histone H3(K9) Universal Methyltransferase Assay Kit is designed for the detection of histone H3(K9) methyltransferase (HMT) activity in screening and profiling applications. The key to the Histone H3(K9) Universal Methyltransferase Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, purified G9a, SUV39H1 and SUV39H2, and reagents for 100 enzyme reactions.

COMPONENTS:

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Dillon S.C., et al. 2005. Genome Biology 6:227.

Application Reference(s):
DNA methyltransferase 3a regulates osteoclast differentiation by coupling to an S-adenosylmethionine-producing metabolic pathway (2015)

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit