Human SAP145, a substrate for PRMT9, also known as Splicing Factor 3b Subunit 2 (SF3B2), (GenBank accession No. NM_006842), (a.a. 401-550) with an N-terminal GST-tag, MW= 45kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Q13435

SAP145, Splicing Factor 3b Subunit 2, SF3B2m, SAP

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 16mM glutathione, 20% glycerol

Aqueous buffer solution

At least 6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Yang, Y., et al. (2015) Nat Commun 6, 6428

2. Falk, S., et al (2016) Nat Commun 7, 13573

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Methyltransferase

Protein