The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8 activity for screening and profiling applications. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

protein arginine N-methyltransferase 8, PRMT8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4

The PRMT8 Chemiluminescent Assay Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethio

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS:

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and HTS applications.

Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit