The PRMT7 Homogeneous Assay Kit (Histone H2b) is designed to measure PRMT7 activity for screening and profiling applications. PRMT7 methylates arginine residues on various protein substrates and is involved in DNA transcription, RNA splicing, DNA repair, cell differentiation, and metastasis.The key to the PRMT7 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with the substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Protein Arginine Methyltransferase 7, [Myelin Basic Protein]-Arginine N-Methyltransferase PRMT, Histone-Arginine N-Methyltransferase PRMT7, Protein Arginine N-Methyltransferase 7, EC 2.1.1.321, KIAA1933, EC 2.1.1, SBIDDS

The PRMT7 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with His-tagged PRMT7 substrate, primary antibody, methylation assay buffer, and purified PRMT7 for 384 enzyme reactions.

Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

See assay protocol for detailed instructions.

At least one year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

You Feng, et al. 2014. J Biol Chem 289:32604.

Avoid multiple freeze/thaw cycles.

Methyltransferase

Assay Kit