The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The NSD3 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified NSD3 enzyme for 96 enzyme reactions. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

WHSC1L1, Protein Whistle, NSD3, Nuclear SET Domain-Containing Protein 3, Wolf-Hirschhorn syndrome candidate 1-like 1, NSD

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS:

See product datasheet for detailed protocol.

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

MATERIALS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit