The NSD2 Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

• TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)

• Luminometer or fluorescent microplate reader capable of reading chemiluminescence

• Adjustable micropipettor and sterile tips

• Rotating or rocker platform

WHSC1, Wolf-Hirschhorn Syndrome Candidate 1, MMSET, Multiple Myeloma SET Domain Containing Protein, NSD2, Nuclear SET Domain-Containing Protein 2, REIIBP, WHS, TRX5, NSD

The NSD2 Chemiluminescent Assay Kit comes in a convenient format, with 384-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransfer

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

Stable at least one year from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and HTS applications.

Dillon, S.C., et al. Genome Biology 2005; 6:227.

Bennet, R.L., et al. Cold Spring Harb Perspect Med. 2017; 7(6): a026708.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit